Just as investigators believed they were approaching a major breakthrough, the disappearance of Nancy Guthrie took another unexpected turn. A black glove recovered from desert brush near her Tucson-area home initially appeared to be a pivotal piece of evidence. The item closely resembled the gloves worn by a masked individual captured on surveillance footage outside the 84-year-old’s residence shortly before she vanished. For a moment, it seemed like the forensic clue authorities had been waiting for. However, recent updates have added new layers of complexity rather than clear answers.
According to statements from the Pima County Sheriff’s Department, DNA collected from the glove was submitted to the FBI’s Combined DNA Index System (CODIS), a national database used to compare profiles from crime scenes with those of known offenders and missing persons. The results, however, produced no matches. Investigators also confirmed that the DNA found on the glove does not match other DNA previously recovered from inside Nancy’s home. This means authorities are now working with at least two separate DNA profiles, both currently unidentified. While forensic testing remains one of the most powerful investigative tools available, officials caution that results can take time and do not always deliver immediate conclusions.
Throughout the investigation, law enforcement agencies have continued to examine surveillance footage, digital data, and physical evidence. Video from a doorbell camera showed a masked person near the home carrying a distinctive backpack. Officials later released updated descriptive details about a person of interest in hopes of generating additional tips. Meanwhile, more than 13,000 pieces of information have reportedly been submitted to investigators, and a reward of up to $100,000 has been offered for credible information leading to Nancy’s location or the arrest and conviction of anyone involved. Authorities continue to review each lead carefully.
Amid widespread public attention and online speculation, Sheriff Chris Nanos publicly stated that Nancy’s family members have been cooperative and have been cleared as suspects. He emphasized the importance of compassion during an active investigation, reminding the public that the family is experiencing a deeply difficult time. The case remains open and active, with additional forensic analysis underway. While the glove discovery once seemed poised to unlock definitive answers, it has instead deepened the mystery. Investigators maintain that every piece of evidence matters, and they remain committed to pursuing all available avenues in the ongoing search for Nancy Guthrie.
